![]() Adjust the pH to 5.5 with glacial acetic acid (about 110mL). The final volume should be 1 liter.īuffer P3 - Neutralization Buffer for Qiatips, Midiprep, Maxiprep, and Gigaprep kits.ĭissolve 294.5g potassium acetate in 500mL dH 2O. Add 100mg RNase A per liter of P1.ĭissolve 8.09g of NaOH pellets in 950mL dH 2O, 50mL 20% SDS solution. Prep - Dissolve 6.06g Tris base, 3.72g EDTA-2H 20 in 800mL dH20. Storage condition - 4 oC after adding RNase A If using Tris-HCl reagent, the qualities used should be recalculated.ĥ0mM Tris-Cl, pH 8.0, 10mM EDTA, 100ug/mL RNase A Buffer calculations are base on Tris base adjusted to pH with HCl (Tris-Cl). Do not autoclave isopropanol-containing buffer, but sterilize by filtration instead. Note: Buffer compositions are given per liter of solution. Qiagen Plasmid Prep - Buffer Composition Qiagen Plasmid Prep - Buffer Compositon and Preparation
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